FIG. 3.

GerBA in spore lysates. Lysates from equal amounts (15 OD₆₀₀ units) of spores of strains PS832 (wild type; lane A), FB60 (ΔgerB; lane B), FB86 (ΔgerA ΔgerK; lane C), and FB72 (ΔgerA ΔgerB ΔgerK; lane D) were run on an SDS-PAGE gel and transferred to an Immobilon membrane, and GerBA was detected as described in Materials and Methods. The arrowhead points to GerBA bands in region II as described in the legend to Fig. 2. Background bands and molecular mass markers are labeled as in Fig. 2. Note that the samples run in Fig. 2 were from spores that overexpressed GerBA while the samples run in this experiment were from spores that did not overexpress GerBA. That difference and the low exposure level of the Western blot shown here account for the relative lack of higher molecular mass GerBA observed in this figure compared to Fig. 2 and 6.