Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 2;20:446. doi: 10.1186/s12967-022-03649-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — EMCN in ECs does not affect tumor growth or angiogenesis and aggravates spontaneous lung metastasis from subcutaneous syngeneic tumors. A The schematic diagram shows the experimental design of tamoxifen induction, subcutaneous implantation of LLC tumor cells and tumor harvest. B LLC cells were subcutaneously injected into WT and EMCN^ecko mice for 14 days. LLC tumors were dissected and photographed (left). Tumor weight was measured by a precision balance (right, data represent the mean ± SD, n = 10 mice per group). Statistical analysis was performed using unpaired Student’s t test. **p < 0.01. C Representative CD31 staining and HE images of tumor sections from WT and EMCN^ecko mice (left panel) are shown. CD31 indicates vascular density. Quantification of CD31 expression was performed (right panel). (Data represent the mean ± SD, n = 4). Statistical analysis was performed using unpaired Student’s t test. ns: not significant. Scale bars: 100 μm. D Representative Ki67 staining images of tumor sections from WT and EMCN^ecko mice are shown (left panel). Quantification of Ki67 expression was performed (right panel) (data represent the mean ± SD, n = 4). Statistical analysis was performed using unpaired Student’s t test. Ns, not significant. Scale bars, 100 μm. E The schematic diagram shows the experimental design of tamoxifen induction, intravenous injection of LLC tumor cells and pulmonary analysis. F Representative images of lungs harvested from WT and EMCN^ecko mice after 21 days of LLC tumor implantation (left, n = 6). Metastatic foci were quantified (right, data represent the mean ± SD, n = 6). Statistical analysis was performed using unpaired Student’s t test. *p < 0.05. G Overall survival of intravenous pulmonary metastasis in WT and EMCN^ecko mice. EMCN^ecko (n = 7), WT (n = 8). The EMCN^ecko mouse groups showed worse overall survival (p = 0.0102). Statistical analysis was performed using the log-rank test. H The schematic diagram shows the experimental design of tamoxifen induction, subcutaneous implantation of LLC tumor cells, tumor resection and lung analysis at different time points. I Representative HE-stained images of lung sections from WT and EMCN^ecko tumor-bearing mice at different time points after surgery. The left panel shows lung metastasis images, and the right panel shows HE-stained sections