Table 1.

Timeline of Polymerase Chain Reaction (PCR) for monkeypox virus and virus isolation.

Days from symptoms onset	Oropharyngeal swab		Ulcerated perianal lesion		Anal swab		Foot lesion		Plasma		Seminal fluid		Urine
	PCR cycle threshold	Viral Isolation	PCR cycle threshold	Viral Isolation	PCR cycle threshold	Viral Isolation	PCR cycle threshold	Viral Isolation	PCR cycle threshold	Viral Isolation	PCR cycle threshold	Viral Isolation	PCR cycle threshold	Cell culture
9	23	Positive	20	Positive	17	Positive	14	Positive		/	n.a.	/	n.a.	/
10	36	Negative	20	Positive	n.a.	/	n.a.	/	33	Negative	31	Negative	Negative	/
11	33	Negative	22	Positive	n.a.	/	n.a.	/	33	Negative	n.a.	/	n.a.	/
12	33	Negative	n.a.	/	22	Positive	n.a.	/	34	Negative	n.a.	/	n.a.	/
15	Negative	/	n.a.	/	Negative	/	n.a.	/	Negative	/	n.a.	/	Negative	/
19	35	a.o.	n.a.	/	Negative	/	n.a.	/	Negative	/	n.a.	/	Negative	/

List of abbreviations: n.a, not available; a.o, analysis ongoing; Polymerase Chain Reaction, PCR.