Fig. 5. Correlation of relevant phenotypes with 5-fluorotryptophan production: percentage of 5-fluorotryptophan appearance as a function of biomass (as measured by crystal violet staining) (A), clustering (as measured by changes to optical density at 600 nm) (B), curli production (as measured by total green fluorescence) (C), and relative metabolic activity (as measured by resorufin fluorescence) (D), for E. coli MC4100 cultures following 48 h of incubation in 100 mM phosphate buffer at pH 7 in the absence (lower white dot) and presence of 0.05 mg mL⁻¹ of P1 (black solid dot) and functional polymers P1-mod-aldehyde (coloured dots), followed by incubation with reaction buffer for another 24 h. Where relevant, data for E. coli PHL644 cultures is shown (top white dot). Median and 25th to 75th percentiles shown for at least 4 replicates.