Table 4. qPCR result as a function of the existence, or not, of a skin lesion compatible with TL.

qPCR (parallel analysis)*	Skin lesion
	Yes n (%)	No n (%)	Total n (%)
Positive	24 (12.6)	167 (87.4)	191 (100)
Negative	9 (12.7)	62 (87.3)	70 (100)
Total	33 (12.6)	229 (87.4)	262** (100)

*Parallel analysis means that every type of biological sample was analyzed in parallel: when all qPCR results were negative, the overall “qPCR” result was negative; when at least one qPCR result in any biological sample was positive, the overall “qPCR” result was positive; **it was not possible to obtain qPCR results of 10 animals.