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. 2022 Oct;33(10):1876–1890. doi: 10.1681/ASN.2022020139

Figure 3.

Figure 3.

Donor and recipient kidney macrophages show a kinetic expression of CCL8 post-transplantation. (A) CCL8 expression on donor kidney resident macrophages (F4/80HIMHC-IIHI cells) from naive untransplanted kidneys. (B) CCL8 expression on donor or recipient kidney macrophages 7 days post-transplantation. The left FACS plot shows intragraft donor (CD45.2+) versus recipient (CD45.1+) CD11b+ cells on day 7 post-transplantation. The middle FACS plots show the expression of F4/80 and MHC-II on CD45.2+ donor (upper plot) and CD45.1+ recipient (lower plot) myeloid cells. The right histograms show the expression of CCL8 by donor (upper histogram) and recipient (lower histogram) F4/80+MHC-II+ macrophages. (C) Quantification of CCL8 expression by donor or recipient kidney macrophages on the indicated days post-transplantation. Mean fluorescence intensity (MFI) of CCL8 by FACS normalized over isotype control was used for CCL8 quantification. n=2–3 per time point. Data are presented as mean±SD. *P≤0.05, calculated using unpaired t test.