Extended Data Figure 10. Regulation and function of Zbtb46⁺ ILC3s.

a. Representative flow cytometry histogram of RORγt expression in CD4⁺ ILCs from the large intestine of indicated mice. Cells were gated from Lineage⁻CD90.2⁺CD127⁺CD4⁺ cells. b,c. Representative flow cytometry histogram of Zbtb46 expression and quantification of Zbtb46 MFI in CD4⁺ ILCs from the large intestine of indicated mice (n = 4 or 5 mice per group). Cells were gated from Lineage⁻CD90.2⁺CD127⁺CD4⁺ cells. d. Representative flow cytometry histogram of RORγt expression in CCR6⁺ ILC3s from the large intestine of indicated mice. Cells were gated from Lineage⁻CD90.2⁺CD127⁺RORγt⁺CCR6⁺ cells. e,f. Representative flow cytometry histogram of Zbtb46 expression and quantification of Zbtb46 MFI in CCR6⁺ ILC3s from the large intestine of indicated mice (n = 4 or 5 mice per group). Cells were gated from Lineage⁻CD90.2⁺CD127⁺RORγt⁺CCR6⁺ cells. Data are representative of two independent experiments in a-f. Data are shown as the means ± S.E.M. Statistics are calculated by two-tailed unpaired Student’s t-test. g. Zbtb46 defines and regulates group 3 innate lymphoid cells that protect the intestine.