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. 2022 Jul 5;55(10):e13289. doi: 10.1111/cpr.13289

FIGURE 7.

FIGURE 7

The effect of scaffold on mouse MC3T3‐E1 osteogenic related genes and protein expression. (A) The effect of different scaffold groups on osteopontin (OPN) protein expression. (B) The effect of different scaffold groups on type I collagen (COL‐I) protein expression. (C) The effect of different scaffold groups on osteocalcin (OCN) protein expression. (D) The effect of the scaffold on the ALP activity (OD 405 nm) of co‐cultured cells on the 3rd and 7th day. ALP activity is homogenized by total cell protein (OD 562 nm). (E) Statistic was showing the effects of different scaffold groups on the mRNA expression of osteogenic differentiation‐related genes, including bone morphogenetic protein 2 (BMP‐2), runt‐related transcription factor 2 (Runx‐2), osteocalcin (OCN), and osteopontin (OPN), type I collagen (COL‐I), Alkaline phosphatase (ALPL) after 10 days of culture. Data are presented as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001.