FIGURE 4.

In vivo fluorescence imaging of P144 distribution. (A, B) Mice were used to generate a uIRIx model and were then injected with 1 mg/kg of the labeled P144 at D21 postsurgery. Fluorescence imaging was performed after intravenous injection of labeled P144 at indicated times. (C, D) Biodistribution of Alexa Fluor 647‐labeled P144 in different organs at indicated times after intravenous injection. Mice were sacrificed at the indicated time after P144 injection and different tissues were isolated for biodistribution evaluation. n = 3–4, ****p < 0.0001. uIRIx, unilateral ischemia–reperfusion injury and contralateral nephrectomy