TABLE 1.
Primers used for RT‐PCR
| Gene | Primer/Probe sequence (5′ to 3′) | GenBank | Size (bp) | Reference primer bank ID/Assay name | |
|---|---|---|---|---|---|
| NES | F | CCTGGGAAAGGGAGA GTACC | NM_006617 | 111 | 27 |
| R | TGGTCCTTCTCCACCGTA TC | ||||
| CD44 | F | CTGCCGCTTTGCAGGTGTA | NM_000610 | 109 | 28 |
| R | CATTGTGGGCAAGGTGCTATT | ||||
| SNAI1 | F | TCGGAAGCCTAACTACAGCGA | NM_005985 | 140 | 301336132c1 |
| R | AGATGAGCATTGGCAGCGAG | ||||
| SNAI2 | F | TGTGACAAGGAATATGTGAGCC | NM_003068 | 203 | 324072669c2 |
| R | TGAGCCCTCAGATTTGACCTG | ||||
| MSX1 | F | CTCCGCAAACACAAGACGAAC | NM_002448 | 391 | 23 |
| R | CACATGGGCCGTGTAGAGTC | ||||
| Hes1 | F | TCAACACGACACCGGATAAAC | NM_005524 | 153 | 325652058c1 |
| R | GCCGCGAGCTATCTTTCTTCA | ||||
| GAPDH | F | AGCAATGCCTCCTGCACCACCAAC | NM_002046 | 137 | 23 |
| R | CCGGAGGGGCCATCCACAGTCT | ||||
| RUNX2 | F | AGGCGGTCAGAGAACAAAC | NM_001015051 | 129 | Hs.PT.56a.19568141 |
| R | CTTCACAAATCCTCCCCAAGT | ||||
| SP7 | F | GGAGCCATAGTGAACTTCCTC | NM_152860 | 94 | Hs.PT.58.3437800 |
| R | AGCTCTCTCCATCTGCCT | ||||
| PPARG | F | GGATTCAGCTGGTCGATATCAC | NM_138711 | 120 | Hs.PT.58.25464465 |
| R | GTTTCAGAAATGCCTTGCAGT | ||||
| LPL | F | CCTTGGAACTGCACCTGTAG | NM_000237 | 148 | Hs.PT.58.45792913 |
| R | GAGAAGCTATCCGCGTGA | ||||
| CHAD | F | TGTTTCAGCGTGGTTACAC | NM_001267 | 109 | Hs.PT.58.40907038 |
| R | ACAATGCCTTCCAGTCCTTT | ||||
| ACAN | F | AGATTCACAGAACTCCAGTGC | NM_001135(2) | 105 | Hs.PT.56a.742783 |
| R | ACCTACGATGTCTACTGCTTTG | ||||
| Col2A1 | F | GTTTTCCAGCTTCACCATCATC | NM_033150 | 121 | Hs.PT.58.4107778 |
| R | CCTCAAGGATTTCAAGGCAAT | ||||
| ACTA2 | F | CACGAAGCTCATTGTAGAAAGAG | NM_001141945 | 115 | Hs.PT.56a.39662523 |
| R | GCACAGAGCAAAAGAGGAATC | ||||
| TUBB3 | F | CCTCCGTGTAGTGACCCTT | NM_001197181 | 89 | Hs.PT.58.20385221 |
| R | GGCCTTTGGACATCTCTTCAG | ||||
| CD24 | F | CAATGTCAAATCCAAAGCCTCA | NM_013230 | 130 | Hs.PT.58.45758278.g |
| R | CTCAACGTATTGTTTCGACAGC | ||||
| CD56 (NCAM1) | F | CCGTCATCCTGCTTGATCAG | NM_181351 | 110 | Hs.PT.58.3970990 |
| R | GAGTTCAAGACGCAGCCA | ||||
| LRRC17 | F | CAAGTCTTCTAATACGCCATAGTCA | NM_005824 | 136 | Hs.PT.58.615298 |
| R | TGAGATGAAACCCTGCAAGTAG | ||||
| KCNK12 | F | AGGTTGAAGAACAGGATGGTC | NM_022055 | 130 | Hs.PT.58.21235707 |
| R | TACTTCGTGGGCACCGT | ||||
| HAPLN1 | F | CAGATTGAAATCAGCACCAGAAG | NM_001884 | 143 | Hs.PT.58.3994973 |
| R | CGCTAGCTTCACTTGATCTCC | ||||
| SUSD2 | F | GCATGATGGAGACCCTGTC | NM_019601 | 89 | Hs.PT.58.1294267 |
| R | GGAGGTGCTGAGCTTCAC | ||||
| PTPRE | F | CTCCCAGACCATTCTCCAGA | NM_130435 | 103 | Hs.PT.58.40307003 |
| R | CAATGCTTCCTACATAGATGGTTAC | ||||
| FBLN2 | F | CCAGGCACTCGTCATTGTC | NM_001998 | 115 | Hs.PT.58.38361266 |
| R | CCAACTCTGTCCATTCTATCCC | ||||
| DLL1 | F | GTCACAAAATCCATGCTGCTC | NM_005618 | 96 | Hs.PT.58.41063402 |
| R | GTGGGGAGAAAGTGTGCAA | ||||
| ACTB | F | CCTTGCACATGCCGGAG | NM_001101 | 110 | Hs.PT.39a.22214847 |
| R | ACAGAGCCTCGCCTTTG |
Abbreviations: ACAN, Aggrecan; ACTA2, Actin Alpha 2; ACTB, Actin Beta; CHAD, Chondroadherin; Col2A1, Collagen Type II Alpha 1; DLL1, Delta Like Canonical Notch Ligand 1; FBLN2, Fibulin 2; GAPDH, Glyceraldehyde‐3‐Phosphate Dehydrogenase; HAPLN1, Hyaluronan And Proteoglycan Link Protein 1; KCNK12, Potassium Two Pore Domain Channel Subfamily K Member 12; LPL, Lipoprotein Lipase; LRRC17, Leucine Rich Repeat Containing 17; MSX1, Msh Homeobox 1; NCAM, Neural Cell Adhesion Molecule 1; NES, Nestin; PPARG, Peroxisome Proliferator Activated Receptor Gamma; PTPRE, Protein Tyrosine Phosphatase Receptor Type E; RUNX2, RUNX Family Transcription Factor 2; SANI2, Snail Family Transcriptional Repressor 2; SNAI1, Snail Family Transcriptional Repressor 1; SUSD2, Sushi Domain Containing 2; TUBB3, Tubulin Beta 3.