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. 2022 Sep;32(9):1727–1735. doi: 10.1101/gr.276647.122

Figure 1.

Figure 1.

RNA composition per sequencing library. Pol II short RNAs include independently transcribed small nucleolar RNAs U3, U8, and U13; small Cajal body–specific RNAs 2 and 17; and spliceosomal RNAs except U6 and U6atac. Pol III short RNAs include transfer RNAs, spliceosomal RNAs U6 and U6atac, small ILF3/NF90-associated RNAs, the RNA component of the RNase P ribonucleoprotein, the RNA component of mitochondrial RNA processing endoribonuclease, the 7SK RNA component of the nuclear ribonucleoprotein, the 7SL RNA component of the signal recognition particle, Ro-associated RNAs, vault RNAs, and brain cytoplasmic RNA 1. Intronic RNAs include small nucleolar RNAs and small Cajal body–specific RNAs, except those transcribed by Pol II, and the MALAT1-associated small cytoplasmic RNA. Short RNA precursors include sequences that align within a 500-bp window upstream of and downstream from transfer RNAs, small nuclear RNAs, small nucleolar RNAs, and small Cajal body–specific RNAs but do not fully align to the mature RNA. The categories sense and antisense comprise transcripts associated with mRNAs and lncRNAs in the sense and antisense orientation, respectively.