Fig 2. Ratio of THP-1 cell infection by L. infantum promastigotes in the absence (negative control) or presence of cytochalasins A-D (20 to 2.5 μM, 2 h incubation).

A) After 2 hours of incubation, the cytochalasin was removed, and late-stage L. infantum promastigotes were added. B) Cytochalasins were not removed after incubation, and late-stage L. infantum promastigotes were added. Phagocytosis inhibition is expressed as a percentage of cells infected after counting 200 random cells. C) The toxicity of cytochalasins to promastigotes is expressed as the remaining living parasites after 24 h of incubation. **** p < 0.0001.