TABLE 7.

Effect of supplementation of pTiC58 virA and virG in restoring the induction of the virE_C58::cat fusion in the octopine Ti plasmid background

Strain	Type	Sp act of chloramphenicol acetyltransferasea (nmol min−1 mg−1 of protein)
		MS control	MS + AS (60 μM)	MS + LS-48 hb
C58(pUCD1553c)	Nopaline	138 ± 5	3,877 ± 354	4,031 ± 381
A348(pUCD1553)	Octopine	154 ± 31	1,555 ± 234	1,248 ± 56
A348(pUCD1553, pBAL23d)	Octopine	200 ± 12	5,244 ± 222	4,745 ± 215
C58(pUCD1553, pBAL23)	Nopaline	215 ± 12	7,442 ± 45	7,695 ± 467

^aValues are means (± standard errors) of six assays. 

^bMS induction medium with 48-h-preincubated tobacco leaf segments. 

^cPlasmid pUCD1553 harbors the virE_C58::cat fusion (59). 

^dPlasmid pBAL23 harbors virA_C58 and virG_C58 in pMH1002 (Table 1; Fig. 1).