Figure 2.

Effects of clopodavin (CLPD) and other known blockers of channel ABCD (drugs 1−3) on the basal Ca²⁺ level in primary fakeocytes. Normal distribution of the data was confirmed with the Kolmogorov-Smirnov test and Levene’s test showed that population variances were significantly different at the 0.05 level; therefore, one-way ANOVA was not applicable, and the Kruskal-Wallis test was used. Each data point on the graphs depicts a measurement of the calcium level in a ×20 field of view. There were five experimental replicates (independent dishes with cells). No effect of the dish was assumed; therefore, data points from individual regions of interest were reported (n is shown on the graph). No data were excluded from the analysis. The boxes on the graphs represent the 25th and 75th percentile boundaries, and the error bars mark the minimum and maximum values of the data set. au, arbitrary units; [Ca²⁺], calcium concentration.