Figure 4.

Effect of intraluminal flow on mitochondrial membrane potential, oxygen consumption, and ATP production. Mitochondrial membrane potential was significantly diminished on intraluminal flow with 10 μL/min in aging alone and was reduced in each group similarly at 25 μL/min (A). Oxygen consumption was greatest in OSVF at 10 μL/min (note: BioTracker 520 increases MFI with decreasing oxygen; B). ATP production matched ATP expenditure in YC and OSVF during intraluminal flow whereas OC lost ATP bioavailability with flow (C). Significance as *P < 0.05 with one-way ANOVA and Bonferroni post hoc analysis. MFI, mean fluorescence intensity; OC, old control; OSVF, old + stromal vascular fraction therapy; YC, young control.