FXYD3 facilitates ion transport across the basolateral membrane of human airway epithelia. A: a representative short-circuit current (Isc) experiment. B: summary data for ouabain-sensitive Isc. Control = 30.61 ± 23.38, FXYD3 siRNA = 21.60 ± 15.89; means ± SD. C: summary data for FXYD3 expression. Control = 1.06 ± 0.42, FXYD3 siRNA = 0.58 ± 0.23; means ± SD. D: summary data for ATP1A1 expression. Control = 1.08 ± 0.49, FXYD3 siRNA = 1.20 ± 0.38; means ± SD. E: summary data for ATP1B1 expression. Control = 1.08 ± 0.46, FXYD3 siRNA = 1.31 ± 0.46; means ± SD. For all graphs, each dot represents the average of technical replicates from 1 human donor (n = 9 donors) and error bars represent the standard error of the mean. P values were calculated using a paired Student’s t test. Isc, short-circuit current; SD, standard deviation.