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. 2022 Sep 26;2022:6459029. doi: 10.1155/2022/6459029

Figure 4.

Figure 4

SOCS1 regulates the tumor suppressor function of miR-331-3p in OS cells in vitro. (a) CCK-8 assay shows that miR-331-3p over-expression inhibits the effect of SOCS1 silencing on cell growth. (b) Colony formation analysis of changes in OS cell proliferation ability. (c) Wound repair experiments show the effect of SOCS1 over-expression on migration ability. (d) The invasion ability of cells after cotransfection with miR-331-3p and SOCS1 is determined by the invasion test. (e) Western blot is used to detect the protein expression level of SOCS1 in OS cells after miR-331-3p alone transfection and miR-331-3p and SOCS1 cotransfection. (f) Detection of SOCS1 mRNA expression level in OS cells after miR-331-3p alone transfection and miR-331-3p and SOCS1 cotransfection by quantitative real-time polymerase chain reaction (qRT-PCR). The results are shown as the mean ± standard deviation (SD) from three independent experiments. ∗∗P < 0.01.