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. 2022 Sep 23;61(5):138. doi: 10.3892/ijo.2022.5428

Figure 1.

Figure 1

Identification of SCCs by a label-retaining system using CFSE. (A) Schematic diagram using a CFSE label-retaining system. (B) Phase-contrast, fluorescence and merge images of single spheres derived from the SK-ES-1 cell line at 1 and 5 days after CFSE labeling. Scale bars, 100 µm. (C) Flow cytometric analyses of CFSE fluorescence in spheres derived from the SK-ES-1 cell line at 1 and 5 days after CFSE labeling. CFSE, carboxyfluorescein diacetate succinimidyl ester; SCCs, slow-cycling cells.