HBSS/protease inhibitor solution

Reagent	Final concentration	Amount
1× HBSS	N/A	0.33 mL per 1.2 g mouse liver (equivalent to 0.275 mL/g of wet tissue)
1× Halt protease inhibitor cocktail	1:100	10 μL/mL of HBSS
Total	N/A	0.337 mL per 1.2 g mouse liver (equivalent to 0.275 mL/g of wet tissue)

Prepare HBSS with protease inhibitor fresh for each mitoDAMP isolation and maintain on ice; do not store.