PBS (Mitochondrial Isolation Kit for Tissue; prepared according to manufacturer’s instructions)

Reagent	Final concentration	Amount
BupH™ Phosphate Buffered Saline	0.1 M sodium phosphate 0.15 M sodium chloride	1 package (included in kit)
Sterile ultrapure water	N/A	500 mL
Total	N/A	500 mL

Sterile filter and maintain at 4°C for long term storage.