Mitochondrial Isolation Kit for Tissue; prepared according to manufacturer’s instructions

Reagent	Final concentration	Amount
Mitochondrial Isolation Reagent C	N/A	1.5 mL per 1.2 g mouse liver (equivalent to 1.25 mL/g of wet tissue)
Sterile ultrapure water	N/A	1.5 mL per 1.2 g mouse liver (equivalent to 1.25 mL/g of wet tissue)
1× Halt protease inhibitor cocktail	1:100	10 μL/mL of Wash Buffer
Total	N/A	3.03 mL per 1.2 g mouse liver

Prepare immediately before use and maintain on ice; do not store.