REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Source of mitoDAMPs – livers or cell lines, as desired
Chemicals, peptides, and recombinant proteins
Pierce Chromogenic Endotoxin Quant Kit	Thermo Fisher Scientific	Cat # A39552
Halt protease inhibitor cocktail	Thermo Fisher Scientific	Cat # 78430
Hanks Balanced Salt Solution (HBSS)	Thermo Fisher Scientific	Cat # 14170112
Critical commercial assays
Mitochondrial Isolation Kit for Tissue	Thermo Fisher Scientific	Cat # 89874
Mitochondrial Isolation Kit for Cultured Cells	Thermo Fisher Scientific	Cat # 89801
Bio-Rad Protein assay kit II with bovine serum albumin standard	Bio-Rad	Cat # 5000002
Experimental models: Cell lines
Hep-G2 human hepatocellular carcinoma (male)	ATCC	Cat # HB-8065; RRID: CVCL_0027
Experimental models: Organisms/strains
Mouse: C57BL/6NCrl, 8–12-week-old	Charles River Laboratories	https://www.criver.com/products-services/find-model/c57bl6-mouse?region=24
Other
Ultrasonic Processor Sound-Abating Enclosure	Cole-Parmer	Cat # UZ-04712-50
Sonic Dismembrator Fisherbrand™ Model 120 (referred to as “Sonicator” in this protocol)	Thermo Fisher Scientific	Cat # FB120110
Avanti J30I Ultracentrifuge	Beckman Coulter	Cat # J-30I
DWK Life Sciences Kimble™ Kontes™ Dounce Tissue Grinder 15 mL using small/looser fitting pestle	Fisher Scientific (of Thermo Fisher Scientific)	Cat # K885300-0015
2 mL conical microfuge tubes (i.e., Eppendorf Safe-Lock Tubes, 2.0 mL)	Eppendorf	Cat # 022363352
15 or 50 mL conical sterile polypropylene centrifuge tubes	Thermo Fisher Scientific	Cat # 339650 or 339652