Blocking solution
| Reagent | Final concentration | Amount |
|---|---|---|
| Goat serum | 5% | 500 μL |
| BSA | 1% | 0.1 g |
| DMSO | 1% | 100 μL |
| PBT | 1× | Bring up to 10 mL |
| Total | N/A | 10 mL |
Blocking solution
| Reagent | Final concentration | Amount |
|---|---|---|
| Goat serum | 5% | 500 μL |
| BSA | 1% | 0.1 g |
| DMSO | 1% | 100 μL |
| PBT | 1× | Bring up to 10 mL |
| Total | N/A | 10 mL |