Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 30;219(12):e20211476. doi: 10.1084/jem.20211476

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Imianowski et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Expression of Bach2 in developing and mature NK cells. (A) Flow cytometry of Bach2^tdRFP expression by NK cell progenitors within BM (left) and replicate measurements (right). (B and C) Gating strategy for progressive NK cell maturation states from the BM (B) and spleen (C). (D) Bach2^tdRFP expression (left) and replicate measurements (right) in NK cell subsets defined in B from mice of indicated genotypes. (E) Bach2^tdRFP expression (left) and replicate measurements (right) in the NK cell subsets defined in C from mice of indicated genotypes. (F) Immunoblot analysis of BACH2 expression in NK cell subsets sorted from Bach2^Flag animals using an anti-Flag antibody. Numbers show relative expression when compared to an anti–β-actin control. (G) Flow cytometry of Bach2^Flag expression in subsets of NK cells from the spleen (left) and replicate measurements (right). Bach2^tdRFP expression is plotted as ΔMFI (mean fluorescence intensity), calculated as the difference between RFP signal in samples from Bach2^tdRFP/+ animals compared to the mean RFP signal from WT controls (D–E). BACH2^Flag expression is plotted as ΔMFI, calculated as the difference between anti-Flag signal in Bach2^Flag animals and the mean WT control signal. Data representative of two independent experiments with four to six mice per group (A–E and G). ns, not significant (P > 0.05); *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001. Ordinary one-way ANOVA (D, E, and G). Bars and error are mean and SEM.