Table 2.
Relative allele frequencies of the cytochrome b mutations in 21 buffalo-derived and 19 cattle-derived T. annulata resistant isolates collected from the endemic region of Pakistan. Each isolate was characterised by susceptible [S129 (AGC), P253 (CCT) and L262 (TTA)] and resistance mutations [129G (GGC), 253S (TCT), 262S (TCA) and 129G (GGC)/253S (TCT)].
| Field isolates | Total no of Illumina MiSeq reads | Susceptible reads | Resistance reads | Susceptible reads % [S129(AGC), P253 (CCT), L262(TTA)] | Resistant reads % [129G(GGC)] | Resistant reads % [253S(TCT)] | Resistant reads % [262S(TCA)] | Resistant reads % [129G(GGC)/253S(TCT)] | Location |
|---|---|---|---|---|---|---|---|---|---|
| Buffalo | |||||||||
| CY110–B | 18868 | 13699 | 5169 | 72.6 | 27.3 | Gujranwala | |||
| CY123–B | 46438 | 42178 | 4260 | 90.8 | 9.17 | Okara | |||
| CY59–B | 19801 | 14110 | 5691 | 71.2 | 28.7 | Gujranwala | |||
| CY49–B | 12313 | 12313 | 100 | Gujranwala | |||||
| CY5–B | 12341 | 12341 | 100 | Gujranwala | |||||
| CY48–B | 16542 | 16542 | 100 | Gujranwala | |||||
| CY4–B | 21578 | 16127 | 5451 | 74.7 | 25.2 | Gujranwala | |||
| CY60–B | 15433 | 15433 | 100 | Gujranwala | |||||
| CY47–B | 17642 | 17642 | 100 | Gujranwala | |||||
| CY254–B | 12256 | 12256 | 100 | Bahawalpur | |||||
| CY256–B | 12892 | 12892 | 100 | Bahawalpur | |||||
| CY257–B | 47942 | 47942 | 100 | Bahawalpur | |||||
| CY260–B | 13314 | 13314 | 100 | Vehari | |||||
| CY266–B | 21823 | 21823 | 100 | Vehari | |||||
| CY269–B | 12378 | 12378 | 100 | Vehari | |||||
| CY278–B | 21004 | 21004 | 100 | Vehari | |||||
| CY317–B | 14988 | 14988 | 100 | Gujranwala | |||||
| CY320–B | 23324 | 23324 | 100 | Gujranwala | |||||
| CY321–B | 24581 | 16842 | 7739 | 68.5 | 7.8 | 19.2 | 4.3 | Gujranwala | |
| CY322–B | 102021 | 73077 | 28944 | 71.6 | 4.3 | 22.7 | 1.3 | Gujranwala | |
| CY323–B | 12175 | 100 | Gujranwala | ||||||
| Cattle | |||||||||
| CY90–C | 65051 | 48644 | 16407 | 74.7 | 16.4 | 6.0 | 2.6 | Gujranwala | |
| CY136–C | 23906 | 18706 | 5200 | 78.2 | 21.7 | Okara | |||
| CY150–C | 28039 | 14904 | 13135 | 53.1 | 12.9 | 33.9 | Okara | ||
| CY6–C | 21001 | 17589 | 3412 | 83.75 | 16.2 | Gujranwala | |||
| CY26–C | 28672 | 16326 | 12346 | 56.9 | 43.0 | Lahore | |||
| CY38–C | 35600 | 2343 | 33257 | 6.5 | 93.4 | Qadirabad | |||
| CY39–C | 45065 | 1999 | 43066 | 4.4 | 95.5 | Qadirabad | |||
| CY40–C | 29245 | 1113 | 28132 | 3.8 | 96.1 | Qadirabad | |||
| CY41–C | 80153 | 2393 | 77760 | 2.9 | 97.0 | Qadirabad | |||
| CY42–C | 58645 | 1402 | 57243 | 2.3 | 97.6 | Qadirabad | |||
| CY43–C | 12398 | 12398 | 100 | Qadirabad | |||||
| CY277–C | 57086 | 51667 | 5419 | 90.5 | 2.1 | 7.3 | Vehari | ||
| CY272–C | 65893 | 60825 | 5068 | 92.3 | 7.6 | Vehari | |||
| CY235–C | 60586 | 47949 | 12637 | 79.1 | 7.1 | 13.7 | Lodhran | ||
| CY268–C | 4483 | 4483 | 76.9 | 23.0 | Vehari | ||||
| CY212–C | 39057 | 1240 | 37817 | 3.1 | 96.8 | Gujranwala | |||
| CY252–C | 5173 | 5173 | 100 | Bahawalpur | |||||
| CY270–C | 79794 | 78497 | 4297 | 98.3 | 1.4 | 3.9 | Vehari | ||
| CY258–C | 11512 | 11512 | 100 | Bahawalpur | |||||