Table 3.

Haplotype diversity and signature of selection at the T. annulata cytochrome b locus. A total number of susceptible and resistant haplotypes from 21 buffalo-derived and 19 cattle-derived T. annulata isolates showed the haplotype diversity and signature of selection at the cytochrome b locus.

	Haplotype distribution						Neutrality tests
Field isolates	Total haplotypes	Susceptible haplotypes	129G(GGC) resistance haplotype	253S(TCT) resistance haplotype	262S(TCA) resistance haplotype	129G(GGC)/253S(TCT) resistance haplotype	Fay and Wu ‘s (H)	Tajima (D)
Buffalo
CY110–B	3	2		1			1.61917	1.95807
CY123–B	4	3	1				1.56652	1.95807
CY59–B	2	1	1				1.70958	2.46226
CY49–B	1		1				N/A
CY5–B	1		1				N/A
CY48–B	1		1				N/A
CY4–B	2	1	1				1.55322	2.23140
CY60–B	1		1				N/A
CY47–B	1		1				N/A
CY254–B	1		1				N/A
CY256–B	1		1				N/A
CY257–B	1		1				N/A
CY260–B	1		1				N/A
CY266–B	1		1				N/A
CY269–B	1		1				N/A
CY278–B	1		1				N/A
CY317–B	1		1				N/A
CY320–B	1		1				N/A
CY321–B	9	4	2	2		1	1.68770*	1.75932*
CY322–B	15	9	3	2		1	1.10119*	0.88492*
CY323–B	1		1				N/A
Total	22	13	6	2		1
Cattle
CY90–C	20	14	2	2	2		1.02457*	−0.58541*
CY136–C	6	5	1				1.26900	0.82865
CY150–C	6	3	1	2			1.39349	0.75819
CY6–C	4	3	1
CY26–C	5	4		1
CY38–C	4	1	3				1.54706*	1.44162
CY39–C	8	3	5				1.74256*	2.00448*
CY40–C	5	2	3				1.69795*	1.64710
CY41–C	4	1	3				1.62083*	1.98644
CY42–C	6	3	3				1.53179*	1.28502
CY43–C	1		1				N/A
CY277–C	9	7	1	1			1.04126	−0.29438
CY272–C	7	4	3				1.57241*	1.52030
CY235–C	13	10	1		2		1.39659	0.51934
CY268–C	2		1	1			1.67822*	2.34668
CY212–C	2	1	1
CY252–C	1		1				N/A
CY270–C	5	3	1	1			1.38874*	1.01660
CY258–C	1		1				N/A
Total	61	40	12	6	3

Statistically significant departure from neutrality determined with the use of simulations of the coalescent at p < 0.05*.