Table 1:
The sequences of primers used in relative quantitative real-time PCR for IFN-γ, TNF-α, IL10, FOXP3, and ActB genes
| Gene | Oligonucleotide sequence (5′ to 3′) | Tm (°C) | Amplicon size (bp) | |
|---|---|---|---|---|
| IFN-γ | F | GACTATGCGATGAGCGTGAT | 58.4 | 109 |
| R | CCGTCGTAGTTAGGGATGAA | 58.4 | ||
| TNF-α | F | TGGCGCGATCACGTCAACA | 59.5 | 91 |
| R | TGCACCGCATCCTCCAATC | 59.5 | ||
| IL10 | F | GGAATATTGCACAATGGGCGC | 61.2 | 112 |
| R | CACAAGGGAACGCCTATCTC | 60.5 | ||
| FOXP3 | F | GAAACAGCACATTCCCAGAGTTC | 62.9 | 100 |
| R | ATGGCCCAGCGGATGAG | 57.3 | ||
| actB | F | ATGTGGCCGAGGACTTTGATT | 59.5 | 111 |
| R | AGTGGGGTGGCTTTTAGGATG | 61.2 |
TM; Annealing temperature, bp; Base pair, F; Forward, R; Reverse