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Agglutination assay procedure. a) 2 μm polystyrene beads conjugated with streptavidin are incubated with biotinylated ACE2, yielding ACE2-functionalized microbeads. b) Functionalized beads are incubated with SARS-CoV-2 pseudovirus within a 1.5 mL test tube on a shaker at 1200 rpm, resulting in microbead agglutination. c) The completed assay is loaded into the imaging chip via micropipette direct injection.
