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. Author manuscript; available in PMC: 2023 Sep 30.
Published in final edited form as: Circ Res. 2022 Sep 14;131(8):673–686. doi: 10.1161/CIRCRESAHA.121.320661

Figure 5: K180R does not change total SR Ca content.

Figure 5:

Total SR Ca content was quantified by integration of NCX current elicited in response to rapid caffeine application. Following four brief membrane depolarizations from −70 mV to +10 mV at 2 Hz to ensure consistent SR Ca loading, caffeine (10 mM) was applied using a rapid solution exchanger for 15 s at a holding potential of −70 mV. Isoproterenol (1 µM) was added to the external solution to activate beta-adrenergic receptors. NCX current integrals were normalized by cell capacitance, a measure of cell size and expressed as pC/pF. A. Representative traces of the NCX currents from 3 groups. B. NCX integral summary data. WT n = 15 cells from 5 hearts; K180R HET n = 17 cells from 4 hearts; Casq2 KO n = 14 cells from 5 hearts. Data displayed as mean ± SD. Data analyzed using a hierarchical statistical model with Bonferroni correction.