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. Author manuscript; available in PMC: 2023 Oct 1.
Published in final edited form as: J Immunol. 2022 Aug 29;209(7):1286–1299. doi: 10.4049/jimmunol.2101104

Figure 1. RNA sequencing identifies STAT3-dependent and -independent states in cDC1s.

Figure 1.

(A) Whole cell lysates from Stat3fl/fl and Stat3Δ/Δ cDC1s treated with IL-10 or PBS for 0.5 hours were subjected to immunoblotting for the indicated proteins (top). Immunoblot results were quantified by densitometry analysis as indicated (bottom). Samples derived from 3 independent experiments, n = 3. (B - D) RNA-seq was performed on Stat3fl/fl and Stat3Δ/Δ cDC1s following treatment with IL-10, poly I:C, IL-10 + poly I:C, or PBS for 6 hours from independent biological samples, n = 3 per genotype and treatment condition. (B) PCA of RNA-seq results. (C) Volcano plot displaying log2 fold change and significance of transcripts from DEG analysis. DEGs were identified using the following cutoff: |FC| ≥ 2 and p < 0.05. (D) Normalized expression of the top 20 DEGs from (C) in Stat3Δ/Δ (left) or Stat3fl/fl (right) cDC1s. * p < 0.05.