Key resources table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-xCENP-A	Straight Lab8,40	N/A
Rabbit anti-POLR1A	Novus Biologicals	Cat#: NBP2-56122
Mouse anti-UBTF, clone 6B6	Abnova	Cat#: H00007343-M01; RRID: AB_607269
Rabbit anti-Histone H3	Abcam	Cat#: ab1791; RRID: AB_302613
Mouse anti-Beta-tubulin E7	Developmental Studies Hybridoma Bank	Cat#: E7; RRID: AB_2315513
Mouse anti-c-myc, clone 9E10	Sigma-Aldrich	Cat#: M4439; RRID: AB_439694
Mouse anti-Ran	BD Biosciences	Cat#: 610340; RRID: AB_397730`
Alexa Fluor 488	Invitrogen	Cat#: A-11008; RRID: AB_143165
Alexa Fluor 568	Invitrogen	Cat#: A-11011; RRID: AB_143157
Goat anti-Rabbit IgG (H&L) Antibody DyLight 800 Conjugated	Rockland Immunochemicals	Cat#: 611-145-002-0.5; RRID: AB_11183542
Donkey anti-Mouse IgG (H&L) Antibody DyLight 680 Conjugated	Rockland Immunochemicals	Cat#: 610-744-002; RRID: AB_1660920
Bacterial and virus strains
XL1-Blue competent cells	Agilent	Cat#: 200249
Chemicals, peptides, and recombinant proteins
Pregnant mare serum gonadotrophin	Calbiochem	Cat#: 367222
Human chorionic gonadotrophin	Sigma-Aldrich	Cat#: CG10
Hoechst 33342	Invitrogen	Cat#: H3570
Vectashield	Vector Labs	Cat#: H-1000
Alexa Fluor 568-dUTP	Invitrogen	Cat#: C11399
Random hexamers	Invitrogen	Cat#: 100026484
Klenow (exo-) polymerase	New England Biolabs	Cat#: M0212S
Blocking reagent	Roche	Cat#: 11096176001
Salmon sperm DNA	Invitrogen	Cat#: AM9680
Aphidicolin	Sigma-Aldrich	Cat#: A0781
BI-2536	Selleck Chemicals	Cat#: S1109
BMH-21	Sigma-Aldrich	Cat#: SML1183
ML-60218	Sigma-Aldrich	Cat#: 557403
MLN-8237	Selleck Chemicals	Cat#: S1133
NMS-873	Sigma-Aldrich	Cat#: SML-1128
Triptolide	Sigma-Aldridch	Cat#: T3652
Critical commercial assays
TnT Sp6-coupled rabbit reticulocyte system	Promega	Cat#: L2080
Protein A Dynabeads	Fisher	Cat#: 10-002-D
NEBNext Ultra II DNA library Prep Kit for Illumina	New England Biolabs	Cat#: E76452
SuperScript III First Strand Synthesis system	Thermo Fisher Scientific	Cat#: 18080051
RNeasy Mini kit	Qiagen	Cat#: 74104
Deposited data
Xenopus laevis ChIP-seq	26,73	GSE153058
Xenopus tropicalis ChIP-seq	26,73	GSE 199671
Xenopus borealis ChIP-seq	This paper	PRJNA848409
Experimental models: Organisms/strains
Xenopus laevis	Nasco	Cat#: LM00535
Xenopus laevis	National Xenopus Resource	Cat#: NXR_0031
Xenopus tropicalis	Nasco	Cat#: LM00822
Xenopus tropicalis	National Xenopus Resource	Cat#: NXR_1018
Xenopus borealis	Nasco	Cat#: LM00698
Oligonucleotides
Primers (FCR monomer sequence amplification, pJET1.2 Sequencing Primers) FWD: CGACTCACTATAGGGAGAGCGGC REV: AAGAACATCGATTTTCCATGGCAG	26	N/A
Primers (X. laevis CENP-A amplification) FWD: CAAGCTTCGAATTCTGCAGTCGACTGCCACCATGAGACCGGGCTCCACTCC REV: GGGTTAATGAGGGACTGGGGTAAGAGCCTCTAGAACTATAGTGAGTCGTATTAC	This paper	N/A
Primers (X. tropicalis CENP-A amplification) FWD: CAAGCTTCGAATTCTGCAGTCGACTGCCACCATGAGGCCTGGGTCTACTCC REV: (GAGTTACTGAGGGGTTGGGGTAAGAGCCTCTAGAACTATAGTGAGTCGTATTAC)	This paper	N/A
Primers (X. borealis CENP-A amplification) FWD: TAAGCACTCGAGGCCATGAGATCGGGGTCCACTCC REV: AATCGTTCTAGAGGCTTACCCCAGTCCCTCATTAACCC	This paper	N/A
Recombinant DNA
Plasmid: Full length X. laevis CENP-A in pCS2+ vector	This paper	N/A
Plasmid: Full length X. tropicalis CENP-A in pCS2+ vector	This paper	N/A
Plasmid: Full length X. borealis CENP-A in pCS2+ vector	This paper	N/A
Plasmid: Full length X. laevis GFP-xHJURP in pCS2+ vector	Straight Lab	ASP1640
Plasmid: Full length X. laevis xCENP-C-myc in pCS2+ vector	Straight Lab	ASP867
Plasmid: FCR monomer4 in pJET1.2	Straight Lab26	N/A
Plasmid: FCR monomer10 in pJET1.2	Straight Lab26	N/A
Plasmid: FCR monomer16 in pJET1.2	Straight Lab26	N/A
Plasmid: FCR monomer19 in pJET1.2	Straight Lab26	N/A
Software and algorithms
FIJI	81	https://imagej.net/software/fiji/
Matlab		https://www.mathworks.com/products/matlab.html
k-mer counting pipeline	26	https://github.com/straightlab/xenla-cen-dna-paper
cd-his-est	74	http://weizhong-lab.ucsd.edu/cd-hit/
Geneious (7.1.4)		https://www.geneious.com/
RepeatMasker 4.0.9	75	http://www.repeatmasker.org
Other