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. 2022 Oct 3;13(10):844. doi: 10.1038/s41419-022-05292-9

Fig. 4. MLN4924 attenuates cytotoxic effect of Jurkat cells through PD-L1 induction.

Fig. 4

A Jurkat cells co-cultured with MLN4924-pretreated cancer cells underwent apoptosis. Sub-confluent H358 cells were treated with MLN4924 (0.5 μM) for 24 h. After medial removal and PBS washing, suspension of Jurkat cells was added and cultured for 24 h. H358 and Jurkat cells were then harvested, separately, for IB with the indicated Abs. B MLN4924-pretreated cancer cells became resistant to the killing by activated Jurkat cells. H358 cells were treated with MLN4924 (0.5 μM) for 24 h, co-cultured with PHA/PMA-activated Jurkat cells with or without anti-PD-L1 antibody (PD-L1 ab) for 24 h, followed by crystal violet staining of viable adhered H358 cells and photography. Shown are mean ± SEM of three independent experiments. p < 0.05 (*), p < 0.01 (**).