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a. Sorting scheme describing which markers and hashtag antibodies were used to sort and identify, respectively, the thymocyte populations used in this analysis. b. UMAP plot showing distribution of single cells based on hashtag labeling. c. UMAP plot showing the identical single cell distribution as b but using bio-informatic analysis to identify similar clusters of cells. d. Representation of hashtag identities across the 15 clusters identified in the data set.
