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. Author manuscript; available in PMC: 2023 Sep 29.
Published in final edited form as: Cell. 2022 Sep 15;185(20):3671–3688.e23. doi: 10.1016/j.cell.2022.08.014

Figure 6. Effect of deleting the RT0 loop on biochemical activities of G2L4 and GII RTs.

Figure 6.

(A and B) Primer extension assays with 3’-blocked 50-nt DNA or RNA templates incubated for 20 min at 37°C.

(C) Terminal transferase assays. The gels (left) show a 20-min time point, and the plots (right) show time courses for gels shown in Figures S6E, S6F.

(D) MMEJ assays using DNA substrates with CCGG-3’ microhomologies.

The Tables shows kinetic paraments calculated as described in the legends of Figures 3 to 5. The numbers to the left of the gels indicate the positions of size markers in a parallel lane.