Figure 1: Ski expression peaks at the climax of metamorphosis during natural frog metamorphosis.

Genomic DNA-free total RNAs were extracted from the intestine and tail of X. tropicalis tadpoles at indicated stages and subjected to qRT-PCR analysis with primers specific for Ski as well as the control gene EF1α, respectively. Ski expression was normalized to that of EF1α and presented as mean + standard error of the mean (S.E.M), relative to the level at stage 56 in the organs, which was set to 1. Statistical analysis was done with ordinary one-way ANOVA. *: p <0.05.