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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Nat Microbiol. 2022 Sep 22;7(10):1605–1620. doi: 10.1038/s41564-022-01226-5

Extended Data Fig. 2. Multiple fluoropyrimidines disrupt the pyrimidine metabolism pathway in E. coli.

Extended Data Fig. 2

(a) A working model for uracil and uridine import and metabolism in bacterial and mammalian cells. Underlined genes are essential for E. coli growth and metabolites with red circles indicate putative 5-FU metabolites. (b-d) Uracil rescues the growth of E. coli BW25113 in the presence of (b) 5-FU, (c) FUDR, and (d) CAP in a dose-dependent manner. M9MM plus glucose was used as the base media with added uracil from 0.1–10 μg/ml. (e-g) A loss-of-function mutation in uracil phosphoribosyltransferase gene (Δupp) rescues the growth of E. coli BW25113 in the presence of (e) 5-FU, (f) FUDR, and (g) CAP. MIC assays performed in M9MM plus glucose. Values in panels b-g are mean±stdev (n=4 biological replicates per group).