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. 2022 Sep 20;9:908639. doi: 10.3389/fmed.2022.908639

Figure 4.

Figure 4

Inhibition of EC senescence suppresses accumulation of αSMA-positive cells and PDGFB expression in the gel implanted on the mouse lung. (A) IF micrographs of fibrin gel supplemented with GFP-labeled healthy or IPAH patient ECs or in combination with treatment with p16INK4 shRNA or ABT-263 and implanted on the NSG mouse lungs for 7 days; GFP-labeled blood vessels and αSMA expression (top) and GFP-labeled blood vessels and PDGFB expression (bottom) in the fibrin gel. Scale bar, 50 μm. Graphs showing integrated fluorescent density of αSMA (left) and PDGFB (2nd, n = 6, mean ± SEM, *p < 0.05), vessel width (3rd, n = 6, mean ± SEM, *p < 0.05), and branching number (right, n = 5, mean ± SEM, *p < 0.05). (B) Graph showing the protein levels of PDGFB measured by ELISA in healthy or IPAH patient PAECs or in combination with treatment with p16INK4 siRNA or scrambled control siRNA (n = 5, mean ± SEM, *p < 0.05). (C) Graph showing the protein levels of PDGFB measured by ELISA in healthy or IPAH patient PAECs or in combination with treatment with ABT-263 (250 nM, n = 5, mean ± SEM, *p < 0.05).