Fig. 1.

Phenotyping of Setaria viridis under different levels of water deficit. a Setaria viridis (accession A10.1) maintained under available water levels of 100%, 50%, 25% and 0%. Rewatering consists of plants maintained at − 1.4 MPa for 24 h and then rewatered to field capacity, which were evaluated 24 h after rehydration. Photographs were taken in two stages: 24 h after the onset of plant stress and 24 h after rewatering. b Matrix water retention curve to determine the relationship between water levels and water soil potential (MPa). c Expression profile of the drought molecular marker genes SvP5CS and SvGolS2. Letters above the bars indicate statistically significant difference between different conditions (P-value < 0.05, Tukey's HSD test)