Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 3;13:5661. doi: 10.1038/s41467-022-33004-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a SEC-MALS analysis of dIG14 estimates a molecular weight corresponding to a dimer (M_w monomer = 9.7 kDa). b Crystal structure of the homodimer interface formed by antiparallel pairing between β-strands 1 and 6 enabled by flipping out of the C-terminal β-strand; the monomer core becomes more accessible and the interface is primarily formed by hydrophobic contacts (right inset). PDB accession code of the dIG14 crystal structure: 7SKP. c dIG14 design model (green) in comparison with the crystal structure (gray, chain B). Sidechain packing interactions in the non-terminal edge β-strands were well recapitulated in the crystal structure (left inset). A shift in β-strand pairing register observed in the crystal structure is highlighted by the two colored arrows (right inset). d The AlphaFold monomer prediction (left) superimposes well with the design model (Cα-RMSD 1.0 Å); while AlphaFold-Multimer (right) correctly predicts the monomer subunits in the crystal structure (Cα-RMSD 0.6 Å, except for the C-terminal disordered β-strand).