TABLE 1.

Concentrations of amino acids in P. gingivalis W50 continuous culture medium, 1.75% BHI broth containing 2% BSA and 4 mM (each) free serine, threonine, and arginine, before inoculation and in spent, cell-free medium

Residue	Amino acid concentration (mM)a
	Before inoculation		In spent medium
	Free form	Peptide form	Free form	Peptide form
Ser	5.1	11.3	0.1	5.4
Thr	5.1	12.2	0.0	6.8
Arg	5.3	7.5	0.1	3.6
Asx	1.1	19.9	0.7	10.1
Glx	1.6	33.4	1.3	16.8
His	0.3	5.9	0.2	3.1
Gly	0.5	7.7	0.6	3.5
Cys	3.1	12.1	2.9	8.2
Ala	1.4	21.9	1.2	13.8
Tyr	0.4	6.5	0.5	3.3
Val	1.2	13.4	1.6	6.5
Met	0.6	2.4	0.3	0.9
Trp	0.4	NDb	0.0	ND
Phe	1.4	8.6	0.7	4.1
Ile	0.9	6.1	0.6	2.4
Leu	3.3	19.9	2.8	10.4
Lys	1.7	19.8	2.4	10.2
Pro	0.2	15.1	7.2	4.4

^aMean of three determinations taken on consecutive days. 

^bND, not determined.