Fig. 3. Causal network analysis identifies induction of neutrophil-active chemokines as a key biological activity of IL-22 in the colonic epithelium.
a Top 10 pathways enriched in transcriptional changes regulated by IL-22 in human colonoids (n = 4) as identified by IPA, b Circos plot showcasing the shared differentially expressed transcripts regulated by the different cytokines in human colonic organoids (purple lines connecting same genes across DEG lists), c Venn diagrams of shared canonical pathways identified in IPA between IL-22 and other pro-inflammatory cytokines, d clique of neutrophil attracting chemokines regulated by IL-22 identified by protein–protein interaction (PPI) network analysis (STRING), colors depict fold changes [loge]. e Rulation of transcripts coding for chemokines by IL-22 and other cytokines in human colonoids, f cumulative effect of IL-22 and IL-17A co-treatment in the expression of neutrophil-attracting chemokines (FDR: false discovery rate). Source data for a, e and f are provided as a Source Data file.