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. 2022 Oct 3;13:5820. doi: 10.1038/s41467-022-33331-8

Fig. 7. IL-22 mediated induction of neutrophil-active chemokines in colonic epithelial cells is abrogated by JAK inhibition.

Fig. 7

a Representative images of immunostaining for IL22RA1, pSTAT3 and MAP3K8 in the colon of a healthy control, UC patient without active inflammation and a UC patients with active inflammation, b, c relative expression of Cxcl1 and Cxcl5 in IL-22 treated colonoids generated from Villin-Cre x Stat3fl/fl mice (Stat3ΔIEL), Map3k8−/− mice and controls, (n = 12 biological replicates, Mann–Whitney test, two-tailed *p < 0.05, **p < 0.01). d Effects of STAT3 genetic disruption in key gene transcripts regulated by IL-22 (GSE15955) e effects of a JAK inhibitor (tofacitinib) in CXCL1 and CXCL5 expression in human colonic organoids (n = 3, biological replicates, ANOVA with Tukey’s multiple comparison test, *p < 0.05, ***p < 0.0001). Source data for b–d and are provided as a Source Data file.