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. 2022 Sep 20;13:892254. doi: 10.3389/fimmu.2022.892254

Figure 1.

Figure 1

LC migration from the epidermis induces immunocompetence associated transcriptional modules. (A) A schematic illustrating isolation of primary human LCs. Split healthy skin was treated with dispase for 20 h to dissociate epidermis. Steady-state LCs were isolated from the epidermis by digestion with liberase TM or migrated from the epidermal sheets for 48 h in cell culture medium (B) UMAP dimensionality reduction analysis of Scran normalised single cell data from steady-state (585) and migrated (387) LCs originating from the same donor. (C) Heatmap displaying the 1002 upregulated DEGs in steady-state LC and 1012 DEGs upregulated in migrated LC (FDR corrected p=<0.01, logFC>1). (D) Gene ontology analysis (Toppgene) results are displayed alongside for steady-state and migrated LC upregulated DEGs (-log10 FDR corrected p-values) (E) Trackplots displaying genes included in ontologies upregulated in steady-state (mRNA metabolic process) and migrated LC (antigen processing and presentation, immune effector process).