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. 2022 Sep 20;13:892254. doi: 10.3389/fimmu.2022.892254

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Copyright © 2022 Davies, Sirvent, Vallejo, Clayton, Douilhet, Keeler, West, Ardern-Jones, MacArthur, Singh and Polak

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Migration of LCs from the epidermis enhances their immunoregulatory transcriptional programming. (A) Venn diagram displaying the number of genes from tolerogenic gene signature 1 (tol 1), curated from literature exploring genes associated with DC or macrophage tolerogenic function, within the whole LC single cell dataset. (B) Gene Set Variation Analysis (GSVA) displaying enrichment of tol 1 in the LC populations. FDR corrected p-values and logFC are displayed. (C) Violin plots and UMAP marker plots displaying the expression of genes within tol 1 amongst steady-state and migrated LCs (FDR corrected p-values <0.01, logFC>1). (D) Flow cytometry analysis of IDO1 protein expression in steady-state and migrated LC extracted by 48 hour culture of epidermal sheets. n=5 steady-state and migrated independent LCs versus isotype control (grey), n=4 migrated LCs. ***p<0.001.