Table 3.
Primer sequences of rumen microbiota for RT-qPCR.
| Itemsa | Sequence of primers (5′-3′)b | Product size (bp) | References |
|---|---|---|---|
| S. bovis | F: CGATACATAGCCGACCTGAG | 235 | This study |
| R: TAGTTAGCCGTCCCTTTCTG | (NR_042052.1) | ||
| L. fermentum | F: AGCGAACAGGATTAGATACCC | 233 | This study |
| R: GATGGCACTAGATGTCAAGACC | (NR_104927.1) | ||
| B. fibrisolvens | F: CACACCATGGGAGTCGGAAA | 71 | This study |
| R: GACCTGCCTTAGCAAGCTCC | (NR_025981.1) | ||
| M. elsdenii | F: ACCGAAACTGCGATGCTAGA | 129 | This study |
| R: GCCTCAGCGTCAGTTGTC | (NR_029207.1) | ||
| S. ruminantium | F: GAGCGAACAGGATTAGATACCC | 194 | This study |
| R: TGCGTCGAATTAAACCACATAC | (NR_036912.1) | ||
| Total protozoa | F: GCTTTCGWTGGTAGTGTATT | 223 | Reference 12 |
| R: ACTTGCCCTCYAATCGTWCT | |||
| Total bacteria | F: CGGCAACGAGCGCAACCC | 130 | Reference 12 |
| R: CCATTGTAGCACGTGTGTAGCC |
aS. bovis, Streptococcus bovis; L. fermentum, Lactobacillus fermentum; B. fibrisolvens, Butyrivibrio fibrisolvens; M. elsdenii, Megasphaera elsdenii; S. ruminantium, Selenomonas ruminantium.
bF, forward; R, reverse.