FIG. 1.
Fusion gene constructs with the Nuc reporter and expected cell location of final products. P59, lactococcal promoter; sp, signal peptide from the M6 preproprotein (spM6) or from the Usp45 preprotein (spUsp45); nucA, structural gene for staphylococcal NucA; cwaM6, sequence specifying the CWA domain from the M6 preproprotein; t1t2, transcriptional terminators; mcs1 and mcs2, multiple cloning sites; cyt, cytoplasm; mb, membrane; cw, cell wall; md, medium. The vertical black arrows in the amino acid sequences of SPM6 and of SPUsp45 indicate the signal peptidase cleavage sites; numbers in parentheses indicate the global net charge of the five amino acids following the cleavage sites.