FIG. 3.
Expression and targeting of Nuc in L. lactis. Western blottings using anti-NucA polyclonal antibodies were performed on fractionated protein extracts from L. lactis MG1363 cells expressing the fusion genes indicated on top. The strains contained plasmids pVE5516, pVE5514, and pVE5236, which express nucA, spM6::nucA, and spM6::nucA::cwaM6, respectively. P, protoplasts; CW, cell wall; SN, supernatant. Purified NucA was used as a reference, and the positions of molecular mass standards (in kilodaltons) are indicated.