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The fusion product of spUsp45::nucA::cwaM6 accumulates in the membrane fraction and not in the cytoplasm. Cells containing pVE5524 were fractionated into cytoplasm (CYT), membrane (MB), and cell wall (CW), and samples were analyzed by Western blotting, using anti-NucA antibodies. Refer to the text for the nature of nonmatured Nuc.
