Figure 5.

Radiation increased secretion of CD9 and CD81-positive EVs in T98G and U373 cells. 24 h after plating, glioblastoma cells were irradiated with photons or high protons at 0 Gy, 2 Gy or 5 Gy. Directly after irradiation, medium was replaced by 2 mL of EV-free culture medium. Supernatant was collected 72 h after irradiation and number of CD9- (A) or CD81-positive (B) objects was determined by using IFCM analyses. N = 4-6. *, ** and *** above the bars indicate significance to respective non-irradiated controls until otherwise indicated. *p < 0.05; **p < 0.01; ***p < 0.001.