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Effects of HEO on mRNA expressions of PI3K (A) and Akt (B), and protein expression of PI3K/p-PI3K, Akt/p-Akt in hippocampus. Immunofluorescence images of PI3K (green), p-PI3K (red), and DAPI (blue) (C); immunofluorescence images of Akt (green), p-Akt (red), and DAPI (blue), Merged images were shown in the right panel (400×) (E); Quantification analysis of (D) and (F). Data were expressed as mean ± SEM (n = 3). # p < 0.05, ## p < 0.01 vs control group; *p < 0.05 vs the CUMS group.
